Current panel name: Red_Clover_DArTag_BI_Cornell_University (1.0)
Panel name for soft release: RCloverDArTagBICUv1
Version description: (1.0) original 250 bp insert size
Availability: Yes
Red Clover
Description: Red clover panel version 1 for 3,000 marker loci developed by Breeding Insight at the University of Florida’s Institute of Food and Agricultural Sciences in collaboration with DArT and funded by USDA-ARS for public use. DArTag assay uses custom designed oligos to amplify targeted SNPs, and their flanking sequences, prior to Next Generation Sequencing (NGS). The sequenced amplicons are demultiplexed and targeted SNPs/haplotypes are analyzed using DArT P/L’s proprietary pipeline. The marker loci were selected from a diverse set of 143 North American red clover, as well as published Eurasian QTLs associated with drought resilience, freezing tolerance, and disease resistance. The panel is suitable for genotyping diploid red clover for breeding decisions and selection. Usage of the panel outside of Trifolium pratense is likely to result in higher missing data and fewer data points. Access to the panel can be found on DArT’s website (https://www.diversityarrays.com/services/targeted-genotying/).
Results and Data Sharing: DArT offers multiple data output formats. Users are encouraged to work with DArT to ensure the data type they desire is provided. Users are also strongly encouraged to request the Missing Allele Discovery Counts (MADC) file from DArT when placing orders. This file contains the read counts of each 109-bp microhaplotype detected in each sample and at each locus. Breeding Insight requests that the MADC file and sample metadata also be sent to bi-genotyping@ufl.edu to be added to the public microhaplotype database for fixed allele naming and public data sharing. Proprietary sample metadata can be privatized in the database for IP protection.
Citation: In preparation. Please contact Breeding Insight for the most up-to-date citation information.
