Current panel name: Blackberry_DArTag_BI_Cornell_University (1.0)
Panel name for soft release: BberryDArTagBICUv1
Version description: (1.0) original 125 bp insert size
Availability: Yes
Blackberry
Description: Blackberry panel version 1 for 3,014 marker loci developed by Breeding Insight at Cornell in collaboration with DArT and funded by USDA-ARS for public use. DArTag assay uses custom designed oligos to amplify targeted SNPs, and their flanking sequences, prior to Next Generation Sequencing (NGS). The sequenced amplicons are demultiplexed and targeted SNPs/haplotypes are analyzed using DArT P/L’s proprietary pipeline The marker loci were selected from a diverse set of 213 cultivated and wild blackberry and Rubus parents and founders across all growth habit types from North American breeding programs. Additional 103 predefined QTL were included. The panel is suitable for genotyping Rubus for breeding decisions and selection. Usage of the panel outside of Rubus is likely to result in higher missing data and fewer data points. Access to the panel can be found on DArT’s website (https://www.diversityarrays.com/services/targeted-genotying/).
Results and Data Sharing: DArT offers multiple data output formats. Users are encouraged to work with DArT to ensure the data type they desire is provided. Users are also strongly encouraged to request the Missing Allele Discovery Counts (MADC) file from DArT when placing orders. This file contains the read counts of each 109-bp michrohaplotype detected in each sample and at each locus. Breeding Insight requests that the MADC file and sample metadata also be sent to bi-genotyping@ufl.edu to be added to the public microhaplotype database for fixed allele naming and public data sharing. Proprietary sample metadata can be privatized in the database for IP protection.
Citation: In preparation. Please contact Breeding Insight for the most up-to-date citation information.
