Sweetpotato

Current panel name: SweetPotato_DArTag_BI_Cornell_University (1.0)

Panel name for soft release: SweetPotato_DArTag_BI_Cornell_University (1.0)

Version description: (1.0) original

Availability: Yes

A harvester pulls a cluster of sweet potatoes from the ground by hand

Description: DArTag Sweet potato panel version 1 for 3120 marker loci developed by Breeding Insight at Cornell University, in collaboration with DArT and funded by USDA-ARS for public use. DArTag assay uses custom designed oligos to amplify targeted SNPs, and their flanking sequences, prior to Next Generation Sequencing (NGS). The sequenced amplicons are demultiplexed and targeted SNPs/haplotypes are analyzed using DArT P/L’s proprietary pipeline.

The marker loci were selected from a diverse set of 48 cultivated sweetpotato parents and founders from North American breeding programs. The panel is best suited for genotyping hexaploid sweetpotato for breeding decisions and selection with germplasm from North America. Its usage in African and Asian breeding materials and samples outside of Ipomoea batatas has not been assessed but could result in higher missing data or few polymorphic data points. Access to the panel can be found on DArT’s website (https://www.diversityarrays.com/services/targeted-genotying/).

Results and Data Sharing: DArT offers multiple data output formats. Users are encouraged to work with DArT to ensure the data type they desire is provided. Users are also strongly encouraged to request the Missing Allele Discovery Counts (MADC) file from DArT when placing orders. This file contains the read counts of each 81-bp michrohaplotype detected in each sample and at each locus. Breeding Insight requests that the MADC file and sample metadata also be sent to bi-genotyping@cornell.edu to be added to the public microhaplotype database for fixed allele naming and public data sharing. Proprietary sample metadata can be privatized in the database for IP protection.

Citation: Zhao D, Sandercock AM, Mejia-Guerra KM, Mollinari M, Wadl, Heller-Uszynska K, Wadl PA, Webster SA, Beil CT, Sheehan MJ (2024). A public mid-density genotyping platform for hexaploid sweetpotato (Ipomoea batatas [L.] Lam). Genes (15, 1047), 1-13 doi: https://doi.org/10.3390/genes15081047

**Disclaimer: These materials are based upon work supported by the U.S. Department of Agriculture, under agreement numbers [8062-21000-043-004-A, 8062-21000-052-002-A, and 8062-21000-052-003-A). Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the views of the U.S. Department of Agriculture. In addition, any reference to specific brands or types of products or services does not constitute or imply an endorsement by the U.S. Department of Agriculture for those products or services.**