In anticipation of the upcoming shipment of your DNA samples to Diversity Arrays Technology (DArT) in Australia, we’d like to ensure you have everything you need. Prepping, packing and shipping your samples correctly will:
- Cut down on the time that your package spends at customs.
- Decrease the possibility that your package gets returned to you.
- Decrease the possibility that valuable samples are lost because of spillage or mispacking.
Plasticware
Make sure you have the approved plasticware. If you’re not sure what to use, please contact Breeding Insight at bi-genotyping@cornell.edu.
All samples need to be prepared in approved plasticware (See the “Plasticware Recommendations” Table).
Plasticware Recommendations
gDNA Sample Requirements
Recommended quantities
For DArT genotyping assays, we recommend:
- 20-30µL of restriction enzyme digestible genomic DNA (gDNA)/free of endonucleases, dissolved in EB Buffer (10mM Tris-Cl, pH8.5) at a concentration of 50-100ng/µL
Other acceptable solvents are:
- Molecular Grade water, and
- Low EDTA TE (10mM Tris pH8, 0.1mM EDTA)
Recommended concentration
DArT assays tolerate up to five fold differences in gDNA concentration, however if the gDNA concentration of the submitted samples is more uniform, they can give you better quality and more directly comparable genotyping data.
DArT may have to charge extra if the gDNA concentration variation exceeds acceptable levels (up to 5 fold) and they need to adjust the gDNA concentration before carrying out the assays.
If you have a few samples with fairly low DNA concentration, we strongly recommend that you group them by standard and low concentration in the second half of the plate.
DArT uses 2µL gDNA of the recommended quality/quantity per assay, therefore:
- For organisms or products listed in the DArT system, please submit at least 20µL of gDNA of required concentration, although 30µL is recommended
- For new organisms or products, please submit a minimum of 30µL of gDNA of required concentration, however higher volumes up to 50µL are welcome
*Important*
We recommend you do not exceed 70µL gDNA as it significantly increases the risk of sample cross-contamination during shipment and handling.
For gDNA Quality Requirements, see info on the DArT website: https://help.diversityarrays.com/docs/gdna-quality-requirements
