Sweetpotato Weevil

Current panel name: SweetPotatoWeevil_DArTmp_BI_CU (2.0)

Panel name for soft release: SwPotWeeDArTmpBICUv2

Version description: (2.0) 100 bp insert size

Availability: Yes

Picture of a sweetpotato weevil

Description: The Sweetpotato Weevil (SPW) 400-loci DArTag panel was developed through a collaboration between DArT and Breeding Insight at Cornell University and funded by USDA-ARS for public use. The assay utilizes custom-designed oligonucleotides to amplify targeted SNPs and their surrounding flanking sequences (microhaplotypes) prior to Next-Generation Sequencing (NGS). Sequenced amplicons are demultiplexed and analyzed using DArT P/L’s proprietary pipeline (https://www.diversityarrays.com/services/targeted-genotying/).

The 400 markers were selected from the concordant SNPs between a high-confidence dataset of ~1 M SNPs discovered from whole-genome sequencing of 40 diverse Cylas formicarius specimens and a DArTseq SNP set generated from 31 weevil specimens. The panel is designed for high-through genotyping of diploid sweetpotato weevils and is suitable for population genetics, diversity studies, and monitoring biosecurity movements. Usage of the panel on Cylas species outside of C. formicarius has not been formally tested and may result in higher rates of missing data.

Results and Data Sharing: DArT offers multiple data output formats. Users are encouraged to work with DArT to ensure the data type they desire is provided. Users are also strongly encouraged to request the Missing Allele Discovery Counts (MADC) file from DArT when placing orders. This file contains the read counts of each 81-bp michrohaplotype detected in each sample and at each locus. Breeding Insight requests that the MADC file and sample metadata also be sent to bi-genotyping@ufl.edu to be added to the public microhaplotype database for fixed allele naming and public data sharing. Proprietary sample metadata can be privatized in the database for IP protection.

Citation: In preparation. Please contact Breeding Insight for the most up-to-date citation information.

**Disclaimer: These materials are based upon work supported by the U.S. Department of Agriculture, under agreement numbers [8062-21000-043-004-A, 8062-21000-052-002-A, 8062-21000-052-003-A, and 8062-21000-052-020-A). Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the views of the U.S. Department of Agriculture. In addition, any reference to specific brands or types of products or services does not constitute or imply an endorsement by the U.S. Department of Agriculture for those products or services.**